Journal: Animals : an open access journal from MDPI

Article Title: New Insights into the Effects of Microbial Muramidase Addition in the Diets of Broiler Chickens.

doi: 10.3390/ani13081356

Figure Lengend Snippet: Figure 3. The morphometric analytic data of immunostained CD3 and CD20 cells in the spleen of different experimental groups. Means with different superscripts (a, b, c, d) are significantly different at p < 0.05.

Article Snippet: Slides were treated with mouse anti-Chicken CD3, clone CT-3 (Bio-Rad Lab., Dubai, United Arab Emirates), and CD20 (ThermoFisher Scientific, Waltham, MA, USA) and examined as described by Amer et al. [36].

Techniques:

Journal: Animals : an open access journal from MDPI

Article Title: New Insights into the Effects of Microbial Muramidase Addition in the Diets of Broiler Chickens.

doi: 10.3390/ani13081356

Figure Lengend Snippet: Figure 4. The immunostained positive CD3 cells (red arrows) and negative cells (black arrows) in the spleen of different experimental groups. (A): MUR0, (B): MUR200, (C) MUR400 and (D): MUR600.

Article Snippet: Slides were treated with mouse anti-Chicken CD3, clone CT-3 (Bio-Rad Lab., Dubai, United Arab Emirates), and CD20 (ThermoFisher Scientific, Waltham, MA, USA) and examined as described by Amer et al. [36].

Techniques:

Journal: Journal of Veterinary Science

Article Title: Protection of chickens against infectious bronchitis virus with a multivalent DNA vaccine and boosting with an inactivated vaccine

doi: 10.4142/jvs.2013.14.1.53

Figure Lengend Snippet: Percentages of CD3+CD4+ and CD3+CD8+ T lymphocytes in the different groups of chickens. Columns are labeled with letters. Different letters indicate statistically significant differences ( p < 0.01) while letters in common indicated that no significant differences ( p > 0.01) were observed in each T cell subgroup.

Article Snippet: The samples (100 µL; 1 × 10 6 cells) was incubated with mouse anti-chicken CD4-PE (BD Biosciences, USA), mouse anti-chicken CD3-SPRD (BD Biosciences, USA), and mouse anti-chicken CD8-FITC (BD Biosciences, USA) primary antibodies (antibody final concentrations as 1.25 µg/100 µL) in the dark at 4℃ for 30 min. After the incubation, all samples were washed three times with cold PBS and then resuspended in 0.5 mL of PBS.

Techniques: Labeling